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Transcriptome Sequencing

Transcriptome sequencing by using Next Generation Sequencing is a fast and reliable tool for genomic knowledge identification. Genephenomics provides the complete analysis of mRNA transcript expression, allowing the discovery of novel genes, identification of novel SNP and InDel, discovery of novel splice variants and chromosomal rearrangements, and identification of fusion genes. Differences in the value of the transcriptome of each sample can be verified with transcriptome sequence.

All living organisms are transcribed in an intermediate called mRNA from DNA sequences to generate protein based on their own genetic data.

If such mRNA is analyzed, you can get information on the genes that have been activated at certain points.

Service overview

 

Reference Based

  • Gene Expression
  • DEG

De novo Based

  • Transcript Assembly
  • Gene Expression
  • DEG

Small-RNA Seq

  • miRNA Expression
  • Identification of miRNA
  • Target Prediction

Main Capture Kit

  • TruSeq Stranded mRNA Sample Prep Kit
  • TruSeq Stranded Total RNA Sample Prep Kit
  • NEXTflex Small RNA-Seq Kit v3

Data Analysis

  • De Novo Sequencing Standard Data Analysis
  • De novo Assembly Statistics
  • Gene Expression Profile Advanced Data Analysis
  • Blastnt or Blastnr
  • Gene Annotations & Ontology Analysis
  • Differentially Expressed Genes (DEG)
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